.406 Ventures is an early-stage venture capital firm that focuses on seed and Series A investments in enterprise-focused companies within the healthcare, data + AI, and cybersecurity sectors. Their strategy is thesis-driven, involving extensive market research and engagement with Fortune 100 buyers to identify critical problems and emerging trends, and they aim to be hands-on partners, providing support beyond just capital.
44% of their portfolio is in Healthcare. Deal activity increased 33% year-over-year (4 deals in the last 12 months). Average disclosed round size is $33.4M (across 32 rounds with reported amounts).
Portfolio
34
Fund Size
$1.1M
Top Stage
Series A
Last 12 Mo
4
Portfolio Breakdown
Stage Distribution
Sector Distribution
Investment Activity
DealsAvg Round Size
Portfolio
34 investments
| Company | Round | Amount | Date |
|---|---|---|---|
 Big Health | Unknown | $23.7M | Feb 2026 |
 Valerie Health | Series A | $30M | Dec 2025 |
 FamilyWell Health | Series A | $8M | Nov 2025 |
 Diana Health | Series C | $55M | Sep 2025 |
| NNema Health | Series A | $14.5M | Jan 2025 |
 Indico Data | Series B | $19M | Jun 2024 |
 Wayspring | Unknown | — | May 2024 |
| Diana Health | Series B | $34M | Oct 2023 |
| SSimon Data | Series D | $54M | Aug 2023 |
 Cortica, Inc. | Growth | $75M | Apr 2023 |
 Keebo | Series A | $15M | Oct 2022 |
| OOort | Series A | $11.5M | Oct 2022 |
| TThreatX | Series B | $30M | Aug 2022 |
 Lynx | Series A | $15M | Mar 2022 |
 Equip | Series B | $58M | Feb 2022 |
| Wayspring | Growth | $75M | Sep 2021 |
| Rretrain.ai | Unknown | $7M | Aug 2021 |
| Cortica, Inc. | Series C | $60M | Jun 2021 |
| Rretrain.ai | Series A | $9M | Mar 2021 |
| Equip | Series A | $13M | Feb 2021 |
 Hurdle | Seed | $5M | Jan 2021 |
 River | Seed | $10.4M | Oct 2020 |
 Iora Health | Series F | $126M | Feb 2020 |
| Iora Health | Series E | $100M | May 2018 |
 Wellist | Series A | $10M | May 2018 |
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Top Co-Investors
F-Prime Capital6 shared
Optum Ventures4 shared
Polaris Venture Partners4 shared
CVS Health3 shared
AlleyCorp3 shared
Founders Fund3 shared
HLM Venture Partners3 shared
Khosla Ventures3 shared
Flare Capital Partners3 shared
Longitude Capital2 shared
Aperture Venture Partners2 shared
Deerfield2 shared
GreyMatter Capital2 shared
Blue Venture Fund2 shared
Thrive Capital2 shared
BoxGroup2 shared
General Catalyst2 shared
Sandbox Industries2 shared
Temasek Holdings2 shared
GE Ventures2 shared
Last updated: 12 April 2026